Mahidol University Annual Research Abstracts Mahidol University Annual Research Abstracts, Vol.28, 2001 213 STUDIES ON TELOMERASE ACTIVITY OF

Studies On Telomerase Activity Of Colorectal Cancer Patients

Mahidol University Annual Research Abstracts Mahidol University Annual Research Abstracts, Vol.28, 2001 213 STUDIES ON TELOMERASE ACTIVITY OF COLORECTAL CANCER PATIENTS FOUND IN THAILAND (NO. 566) Voraratt Champattanachai1, Chantragan Srisomsap1, James R. Ketudat-Cairns1,4, Paiboon Punyarit2 and M.R. Jisnuson Svasti1,3 1Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand; 2Department of Pathology, Pramongkutklao College of Medicine, Bangkok 10400, Thailand; 3Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; 4School of Chemistry, Institute of Scince, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Key words: Telomerase, Colorectal cancer, Cell differentiation Telomerase activity is not detected in normal somatic cells but present in germ cells, immortal cells and almost carcinoma cells. Our objective is to determine the levels of telomerase activity compared with the degrees of cell differentiation of cancer tissue. The frozen tissues of 27 Thai colorectal normal and cancer patients, composed of well differentiated adenocarcinoma (9 cases) and moderately differentiated adenocarcinoma (18 cases) were tested for telomerase activity. By comparison between cancer and normal tissues, the results showed that telomerase activity was not detected in the normal tissues . Of the tissues from well differentiated (Grade I)adenocarcinoma, only one case had weak telomerase activity (1 of 9). Most of tissues from the moderated differentiated (Grade II) adenocarcinoma had detectable telomerase activity (11 of 18): weak activity, 6 cases, moderate activity, 3 cases and strong activity, 3 cases. These results indicate that the levels of telomerase activity may play a role in the degree of cell differentiation in colorectal adenocarcinoma. Supported by the Chulabhorn Research Institute and presented at the 26th Conference on Science and Technology of Thailand, Bangkok, 18-20 October 2000, p. 457. DETECTION OF UREA CYCLE DISORDER ARGININOSUCCINATE SYNTHETASE DEFI-CIENCY IN AN INFANT BY HPLC (NO. 567) Kanokporn Boonpuan 1, Phannee Sawangareetrakul1, Chantragan Srisomsap1, Pornswan Wasant3, Rudee Surarit1,4, James Ketudat-Cairns1, M.R. Jisnuson Svasti 1,2 and Somporn Liammongkolkul3 1Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210; 2Department of Biochemistry Faculty of Science, Mahidol University, Bangkok 10400; 3Medical Genetics Unit, Department of Pediatrics Siriraj Hospital, Faculty of Medicine, Mahidol University, Bangkok 10700; Department of Physiology and Biochemistry, Faculty of Dentistry, Bangkok 10400; Thailand. Key words: urea cycle, enzyme deficiency, argininosuccinate synthetase The urea cycle removes unwanted nitrogen resulting from the metabolic breakdown of amino acids. Inherited deficiencies in any of the five enzymes involved results in accumulation of precursors which may lead to coma and high mortality rate. This report describes a 2 month old female Pakistani infant referred to the Department of Pediatrics, Siriraj Hospital in a coma with vomitting and convulsion. Inherited metabolic disorder was suspected. Plasma amino acids were derivatized with PITC using a Waters Picotag system and then analysed by HPLC on a reverse phase C-18 column. The results showed marked elevation of citrulline and glutamine (2961.97 and 1639.40 nmole/ml), confirming the diagnosis of urea cycle disorders, specifically argininosuccinate synthetase deficiency (ASD), which is the first such case described in Thailand. Supported by the Chulabhorn Research Institute and presented at the 26th Annual Conference of Science and Technology of Thailand, Bangkok, 18-20 October 2000. HPLC ANALYSIS OF PLASMA AMINO ACIDS IN HEALTHY THAI SUBJECTS (NO. 568) Phannee Sawangareetrakul1, Chantragan Srisomsap1, Rudee Surarit1,2, Suthipong Pangkanonc3 and M.R. Jisnuson Svasti1,4 1Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand; 2Department of Physiology and Biochemistry, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand; 3Queen Sirikit National Institute of Child Health, Bangkok, Thailand; 4Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Key words : Plasma amino acid, HPLC The detection of normal pattern of amino acids is also helpful for diagnosis of many inborn errors of metabolism. Standard methods include the amino acid analyser and HPLC. HPLC has some adventages, including lower equipment cost, less caustic buffer system and improved separation of certain amino acids. In this study, the plasma amino acid concentrations were determined in 27 healthy subjects by using hplc systems. The subjects were divided into five groups according to their ages: group 1: 0-6 months; group 2: 6-12 months; group 3: 1-3 years;, group 4: 3-6 years and gr 5: 6-12 years. The results showed that no clear differences between different groups. A wide range was observed for most amino acids. Our results are similar to those be printed in other countries, and appear to be quite reliable. Supported by the Chulabhorn Research Institute and presented at the 26th Annual Conference of Science and Technology of Thailand, Bangkok, 18-20 October 2000. MATRIX METALLOPROTEINASE (MMP)LEVELS IN COLORECTAL CANCER TISSUES OF THAI PATIENTS (NO. 569) Pantipa Subhasitanont1, Chantragan Srisomsap1, Rudee Surarit2, Paiboon Punyarit3 and M.R. Jisnuson Svasti4 a1Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand; b2Department of Physiology and Biochemistry, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand; c3Department of Pathology, Pramongkutklao College of Medicine, Bangkok 10400, Thailand; d4Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.



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